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Blur estimation applied on microscope image

Raw confocal image
Original image		 Raw + blurred
image blurred with gaussian with sigma 1.5 to compensate noise effects		 Restored with RL
image restored with richarsdon lucy, using our blur estimation as input psf (no further preblur, estimation 7.5)		 Restored with RL, median prefiltering
image prefiltered with median filter, and then restored with richarsdon lucy, using our blur estimation (on the median prefiltered image) as input psf (sigma = 4.5).
Original
Original image		 Restored with RL
image restored with Richardson-Lucy		 Restored with RL, with preprocessing

image restored with Richardson-Lucy, with preprocessing